Serum Markers for Septicemia Research Paper Example | Topics and Well Written Essays - 2500 words

Serum Markers for Septicemia - Research Paper ExampleIt is cleared that a single biomarker which is ideal though has not been identified but a series particularised studies on individual pathogen mediated sepsis has been studied extensively,. Challenges that are faced during biomarker research lied in the pretermit of uniform protocol and methodology , standardization of the assays and the detection levels to be fixed age wise from commonwealth to population. The reliable detection of Biomarkers piece of tail be useful to thrust a immense diagnostic and vaticination relevance in relation to the management of sepsis. Sepsis is considered as the most important cause of morbidity and mortality. By sepsis we mean infection of the blood and if it is caused by bacteria we term it as bacteremia and if by computer virus it is termed viremia. As such the infection in the blood is not so dangerous but as blood perfuses the important tissues and organs infections can be carried by blood to the kidneys or lungs which can moderate in the end organ failures of a patient leading to death in severe conditions. The master(prenominal) issue is that sepsis often lacks clinical manifestations so if the specific and the sensitive indicators of the infections are collected and analyzed indeed it will create superior out comes in the management of the illness. Immunologically it can be considered as the dysregulation of the solution of the innate immune system. Biomarkers are hence important to signal the presence and severity of sepsis in early(a) conditions for effective prognostic values and importance.(Chiesa,1998)(De Bont,1994)(Manroe,1979)(Squire,1979). It is very important to note that sepsis is not a true disease and just a physiological manifestation of the systemic immune response in innate resistance majority of the studies define sepsis to be a systemic inflammatory response to a bacteria, virus or fungi. In clinical setting several other physiological symt oms must be presented to properly diagnose sepsis. The common treatments of patients suspected with sepsis aims to eradicate the bacteria in the blood or reduce its growth by using broad spectrum antimicrobials. The gold standard of sepsis diagnosis has been traditionally the use of microbial cultures to identify the etymon of illness. The major limitation of using cultures in the length of the time required to develop cultures to identifiable quantities to detect sepsis and the possible icrobes to start the treatment with antimicrobials and this with Bactek culture takes around 48 hours and hence there is a posit of early detection through the detection of serum biomarkers which thus becomes important. Even the cultures might be unreactive under several conditions including the slow growing and non cultivatable organisms present at very low concentrations . So in the light of this problem alternative diagnostic methods using molecular biology tool kits have been developed to ad dress the situation for rapid and automated detection of sepsis. These tests are basically the ELISA tests, time period cytometric detection of the specific marker antigens,immunocolorimetric tests ,FISH methodologies and PCR and amplified PCR techniques and the main ideology was to detect the marker proteins. In parallel to the cultivation of biomarkers which provides a faster and more sensitive diagnostic methods for infectious microbes thus relies on the monitoring of the changes in specific serum protein biomarker concentrations. Biomarker is best defined as a factor or feature that can